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Fig. 3 | Cancer Cell International

Fig. 3

From: FOXC1-induced LINC01123 acts as a mediator in triple negative breast cancer

Fig. 3

LINC01123 positively regulated oncogenic CMIP in TNBC via miR-663a. a Bioinformatics tools disclosed two possible targets of miR-663a. b Expression of CMIP and LAP1 in TNBC cells transfected with sh/NC or sh/LINC01123#1/2. c The interaction sequences between CMIP and miR-663a was exhibited via starBase prediction. d Luciferase reporter assay carried out in TNBC cells co-transfected with indicated reporters (CMIP-WT, CMIP-Mut) and miR-663a mimics or NC mimics. e The abundance of CMIP and miR-663a in Anti-IgG or Anti-Ago2 group was assessed via RIP assay plus qRT-PCR. f RNA pull down assay indicated the enrichment of miR-663a in Bio-CMIP-WT, Bio-CMIP-Mut or Bio-NC groups. g qRT-PCR and western blot examined the mRNA and protein levels of CMIP in MDA-MB-231 and HCC1937 cells under diverse transfections. h qRT-PCR revealed the expression of CMIP expression in TNBC cells transfected with sh/NC, sh/CMIP#1 or sh/CMIP#2. i, j Colony formation and EdU assays conducted in CMIP-depleted cells. k, l Flow cytometry analysis and TUNEL asaay in CMIP-depleted cells. Error bar meant the SD of data from three independent experiments. **P < 0.01

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