Fig. 5
From: FOXC1-induced LINC01123 acts as a mediator in triple negative breast cancer
LINC01123 was transcriptionally promoted via FOXC1. a FOXC1 expression pattern in TNBC cell lines and MCF-10A was reflected by qRT-PCR. b Overexpression or suppression of FOXC1 was effectively conducted, as evidenced by qRT-PCR. c The influence of FOXC1 on LINC01123 expression was determined via qRT-PCR. d, e JASPAR provided FOXC1 motif and also predicted the binding region of FOXC1 in LINC01123 promoter. The sequences of LINC01123 promoter were obtained from UCSC. f ChIP assay followed by qRT-PCR monitored the abundance of LINC01123 promoter in immunoprecipitates induced by antibodies against FOXC1 or IgG. g, h Luciferase reporter assay showed the impact of FOXC1 overexpression or depletion on LINC01123 transcription in TNBC cells. Error bar meant the SD of data from three independent experiments. *P < 0.05, **P < 0.01