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Fig. 3 | Cancer Cell International

Fig. 3

From: LncRNA MEG3 suppressed the progression of ovarian cancer via sponging miR-30e-3p and regulating LAMA4 expression

Fig. 3

The expressions of LAMA4 in ovarian cancer tissues and cell lines. a The mRNA level of LAMA4 was detected by qRT-PCR in healthy and tumorous ovarian tissues. b The mRNA expression of LAMA4 in immortalized healthy ovarian cell lines (IOSE80 and HOSEpiC) and OC cell lines (SKOV3, OVCAR3, and Caov-4). *P < 0.05, **P < 0.01 vs. IOSE80. c The western-blot assay was used to measure the protein level of LAMA4 in healthy ovarian cell lines (IOSE80 and HOSEpiC) and ovarian cancer cell lines (SKOV3, OVCAR3, and Caov-4). **P < 0.01 vs. IOSE80. d The qRT-PCR results of control, NC and LAMA4 OE groups in SKOV3 and OVCAR3 cell lines. **P < 0.01 vs. control group. LAMA4 over-expression was constructed using the pcDNA3.1 vector. Control group is the blank group. NC, Negative control, the cells were transfected with empty plasmids. LAMA4 OE, LAMA4 overexpression, the cells were transfected with LAMA4 over-expression plasmids. e, f The western blot results of control, NC and LAMA4 OE groups in SKOV3 and OVCAR3 cells. LAMA4 over-expression was constructed using the pcDNA3.1 vector. Control group is the blank group. NC, negative control, the cells were transfected with empty plasmids. LAMA4 OE, LAMA4 overexpression, the cells were transfected with LAMA4 over-expression plasmids. *P < 0.05 vs. control

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