Fig. 5
miR-30e-3p reversed the effect of LAMA4 in SKOV3 and OVCAR3 cells. a The scheme illustrating the regulatory association between LAMA4 3′UTR and miR-30e-3p. The binding sequences were predicted by miRDB database. b The relative expression of miR-30e-3p in healthy and cancerous ovarian tissues. c The expression of miR-30e-3p in healthy cell lines IOSE80 and HOSEpiC, and OC cell lines SKOV3, OVCAR3 and Caov-4 cell lines. *P < 0.05, **P < 0.01 vs. IOSE80 cell line. d The relative luciferase activities in wild-type and mutated-type LAMA4 3′UTR group co-transfected with miR-30e-3p mimic or miR-30e-3p NC. The experiments were conducted in 293T cells. 3′UTR + NC, the cells were transfected with pGL3-LAMA4 3′UTR-Wt; 3′UTR + miRNA, the cells were co-transfected with pGL3-LAMA4 3′UTR-Wt and miR-30e-3p mimic. 3′UTR-MU + miRNA, the cells were co-transfected with pGL3-LAMA4 3′UTR-Mut and miR-30e-3p mimic. 3′UTR-MU + NC, the cells were transfected with pGL3-LAMA4 3′UTR-Mut. **P < 0.01 vs. 3′UTR + NC. e qRT-PCR analysis of the levels of miR-30e-3p in SKOV3 and OVCAR3 cells transfected with miR-30e-3p mimic. **P < 0.01 vs. control group. f Western blot analysis of the expression of LAMA4 protein in SKOV3 and OVCAR3 cell lines transfected with miR-30e-3p mimic with or without LAMA4 over-expression. *P < 0.05, **P < 0.01 vs. control group. g The proliferation of SKOV3 and OVCAR3 cells transfected with LAMA4 over-expression plasmids alone or co-transfected with miR-30e-3p mimic was detected by CCK8 assay. *P < 0.05, **P < 0.01 vs. control group, #P < 0.05 vs. LAMA4 OE group. h The invasion of SKOV3 and OVCAR3 cells transfected with LAMA4 over-expression plasmids alone or co-transfected with miR-30e-3p mimic was detected by Transwell invasion assay. *P < 0.05, **P < 0.01 vs. control group, #P < 0.05 vs. LAMA4 OE group. Control group is the blank control. NC, the cells were transfected with empty plasmids. Mimic, the cells were transfected with miR-30e-3p mimic. Mimic + LAMA4 OE, the cells were co-transfected with miR-30e-3p mimic and LAMA4 over-expression plasmids