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Fig. 1 | Cancer Cell International

Fig. 1

From: EZH2 inhibitors-mediated epigenetic reactivation of FOSB inhibits triple-negative breast cancer progress

Fig. 1

Genetic ablation or pharmacological inhibition of EZH2 resulted in FOSB gene expression in TNBC cells. a Heatmap demonstrated the common differentially expressed genes in MDA-MB-231, MDA-MB-436 and MDA-MB-453 cells either treated with 2 μM GSK343, or stably transduced with shEHZ2, compared to untreated controls. b The mRNAs of FOSB in MDA-MB-231 and MDA-MB-436 cells with or without 2 μM GSK343 treatment were determined by real-time PCR assay. c Immunoblot analysis of the indicated proteins in lysates from cells as in (a) with GAPDH as loading controls. d The mRNA levels of FOSB and EZH2 in MDA-MB-231 and MDA-MB-436 cells transfected with con-siRNA or siRNA against EZH2 were determined by real-time PCR assay. e The mRNAs of FOSB and SUZ12 in MDA-MB-231 cells transfected with con-siRNA or siRNA against SUZ12 were determined by real-time PCR assay

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