Fig. 5
FOSB exhibits tumor-suppressor functions in TNBC cells. a The mRNA and protein levels of FOSB from MDA-MB-231 cells stable expression of pcDNA3(Con) or pcDNA3-FOSB were detected by real-time PCR assay and western blot, respectively. b The cell growth curve of MDA-MB-231 cells stable expression of pcDNA3(Con) or pcDNA3-FOSB. c Clonogenic assay of MDA-MB-231 cells stable expression of pcDNA3(Con) or pcDNA3-FOSB. 1000 cells were seeded into each well of a six-well plate, and cultured for about 2 weeks. Cells were stained with crystal violet and then counted. d FOSB KO MDA-MB-231 cells were generated by CRISPR assay and detected by western blot. e The cell growth curve of FOSB WT and KO MDA-MB-231 cells. f Clonogenic assay of FOSB WT and KO MDA-MB-231 cells. 1000 cells were seeded into each well of a six-well plate, and cultured for about 2 weeks. Cells were stained with crystal violet and then counted. g Each nude mouse was subcutaneously injected with 1 × 107 FOSB WT and KO MDA-MB-231 cells for about 4 weeks. Tumour growth was measured using a caliper at the indicated times after injection. n = 5 for each group. ***P < 0.001. h The image shows representative tumor-bearing mice for each group. i Mice tumor weights in each group were measured after mice were sacrificed. **P < 0.01