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Fig. 3 | Cancer Cell International

Fig. 3

From: LncRNA H19 promotes triple-negative breast cancer cells invasion and metastasis through the p53/TNFAIP8 pathway

Fig. 3

Effect of lncRNA H19 depletion on proliferation, migration, invasion and cell cycle arrest of breast cancer cells. a The expression levels of lncRNA H19, p53, and TNFAIP8 (left) and the protein levels of p53 and TNFAIP8 (right) in BT-549 and MDA-MB-231 cells treated with H19-siRNA detected by qRT-PCR and Western blotting analyses. b Cell cycle profiles of BT-549 and MDA-MB-231 cells treated with H19-siRNA detected by flow cytometry assay. c Inhibition of lncRNA H19 reduced clonogenicity of BT-459 and MDA-MB-231 cells detected by colony formation assay. d–e Inhibition of lncRNA H19 repressed migration and invasion of BT-459 and MDA-MB-231 cells detected by Transwell assay. f Protein levels of N-cadherin, Vimentin and Snail, and E-cadherin in BT-459 and MDA-MB-231 cells with treatment of H19-siRNA detected by Western blotting analysis. Measurements were carried out in triplicate, and experiments were repeated three times. Data are presented as mean ± SD. * p < 0.05 and ** p < 0.01

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