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Fig. 5 | Cancer Cell International

Fig. 5

From: LncRNA H19 promotes triple-negative breast cancer cells invasion and metastasis through the p53/TNFAIP8 pathway

Fig. 5

LncRNA H19 functions as a competitive inhibitor of p53 and abrogates its transcriptional inhibition of TNFAIP8. a The targeting relations of lncRNA H19 and p53 were assessed by RNA pull-down assay. Endogenous p53 enrichment was carried out specifically in lncRNA H19 probe detection as compared with the control group. LncRNA H19 was incubated with nuclear extracts, and p53 protein was measured by western blotting analysis. b The targeting relations of lncRNA H19 and p53 were confirmed by RNA pull-down assay. Endogenous p53 was attenuated specifically in lncRNA H19 probe detection treated with H19-siRNA compared with control group. c Relative luciferase activity in pGL3-TNFAIP8-WT-treated BT-549 and MDA-MB-231 cells co-transfected with pGL4.17, siRNA-NC, or H19-siRNA, respectively. Measurements were carried out in triplicate, and experiments were repeated three times. Data are presented as mean ± SD. ** p < 0.01 and *** p < 0.001

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Cancer Cell International

ISSN: 1475-2867

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