Fig. 1

High CHAF1B expression was negatively correlated with prognosis of LUAD patients and regulated cisplatin sensitivity. a Whole proteome chips showed chat there are 657 significantly changed protein, of which 312 significantly up-regulated protein, and 345 significantly down-regulated protein. There are 46 significantly up-regulated ubiquitination enzymes, including 42 E3; b According to the public database, 8 E3 high expressions, including: ARPC1A, AURKA, CDC20, CDCA3, CHAF1B, FBXO22, PPP1R13L, TRIP12, were negatively correlated with the prognosis of patients with lung adenocarcinoma; c PCR results indicated that the expression of CHAF1B, PPP1R13L and CDC20 in A549/DDP was significantly higher than that in A549; d After knocking down CHAF1B, PPP1R13L and CDC20, the proliferation of A549/DDP cells was significantly decreased, and IC50 was significantly down-regulated; e CHAF1B substrate screening. *p < 0.05, ****p < 0.0001