Skip to main content
Fig. 4 | Cancer Cell International

Fig. 4

From: Adipocytes promote tumor progression and induce PD-L1 expression via TNF-α/IL-6 signaling

Fig. 4

Blockade of TNF-α/IL-6 decreased PD-L1 expression. a Levels of TNF-α and IL-6 in the supernatant of differentiated 3T3-L1 adipocytes detected by ELISA. b Representative western blot images of PD-L1 and phosphorylated-NF-κB protein expression in adi-CM-treated B16-F1 cells. Anti-TNF-α antibody and/or anti-IL-6 antibody were used in neutralizing TNF-α and IL-6. c Quantification of PD-L1 and phosphorylated-NF-κB (d) protein expression on B16-F1 cells treated. e Representative western blot images of PD-L1, phosphorylated-STAT3 and phosphorylated-NF-κB protein expression in adi-CM-treated HepG2 cells with the addition of anti-TNF-α antibody and/or anti-IL-6 antibody. f Quantification of PD-L1, phosphorylated-NF-κB (g) and phosphorylated-STAT3 (h) protein expression on HepG2 cells treated. Data are expressed as mean ± SEM, n = 4 (ELISA) and n = 6 (western blot), ***P < 0.001 Vs adi-CM group. #P < 0.05, ##P < 0.01 and ###P < 0.001 Vs IgG group

Back to article page