Fig. 6

Bcl-2 family mediates the enhanced effect of Gefitinib-induced cell apoptosis by FZKA in vivo. a–c Tumor growth was the most suppressed in the combined group treated with FZKA and Gefitinib. Animal model was constructed and tumor volume as well as tumor weight was measured as indicated. ***p < 0.0001; one-way ANOVA. d Bax and Cyt-C was the most induced in the mice tumor tissues treated with FZKA together with Gefitinib. Western blot was performed to detect the protein level of Bax and Cyt-C in the mice tumor tissues treated with FZKA, Gefitinb, or FZKA combined with Gefitinib, respectively. GAPDH was used as a loading control. Densitometric analysis was performed using ImageJ. e Bax and Caspase-3 was overexpressed in the combined group treated with FZKA and Gefitinib. Immunohistochemistry was carried out to measure the expression of Bax and Caspase-3 in mice tumor tissues treated with FZKA, Gefitinib, or FZKA combined with Gefitinib, respectively. ***p < 0.0001; one-way ANOVA