Fig. 1

Expression of Nanos3 in glioblastoma tissues and glioblastoma cell lines. a Whole-cell RNA extracts were further prepared from NHA cells and the following three GBM cell lines: A172, U251, and LN229. The Nanos3 RNA level was determined by RT-PCR analysis. b Whole-cell proteins were extracted from NHA cells and the GBM cell lines A172, U251, and LN229. The Nanos3 protein level was examined by western blot analysis. c RT-PCR analysis determined Nanos3 expression in normal mouse tissues and tumor tissues of the xenograft tumor models. d Western blot analysis determined Nanos3 protein expression in normal brain tissues and tumor tissues of the xenograft tumor models. e Immunohistochemistry data shows Nanos3 expression in normal HMC3 cells, normal brain tissue and GBM cells (A172, U251, and LN229 cells). f Immunofluorescence shows Nanos3 expression in NHA and A172 cells. All experiments were carried out in triplicate. Data are shown as the mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.001