Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Nanos3, a cancer-germline gene, promotes cell proliferation, migration, chemoresistance, and invasion of human glioblastoma

Fig. 2

CRISPR/Cas9-mediated knockdown of Nanos3 in GBM cell lines. a Lenti-sgRNA against Nanos3 and lenti-Cas9 plasmids were transfected into GBM cell lines (A172, U251, and LN229). A schematic overview of the procedure used for the CRISPR/Cas 9 knockdown experiment is shown. b Western blot analysis detected whether cas9 protein was transfected into GBM cells successfully and whether Nanos3 protein was deleted. c The PCR band for the Nanos3-sgRNA infected cells was subcloned and analyzed by sanger sequencing. The sequences of three candidate clones are shown. d RT-PCR assays were utilized to test the RNA expression of the three candidate clones

Back to article page