Fig. 4
NNT-AS1 directly binds to miR-186 and negatively regulates its expression. a, b The expression of miR-186 in DDP-sensitive or DDP-resistant CC tissues and cell lines was detected using qRT-PCR. c The correlation between NNT-AS1 and miR-186 was analyzed. d The predicted binding sequences between NNT-AS1 and miR-186 were shown. e, f Luciferase reporter assays in HeLa/DDP and SiHa/DDP cells after the co-transfected with NNT-AS1-WT or NNT-AS1-MUT and miR-186 mimic or miR-NC were conducted. g The level of NNT-AS1 was measured using qRT-PCR in samples pulled down by biotinylated miR-186 or negative control. h QRT-PCR was used to determine the expression of miR-186 in HeLa/DDP and SiHa/DDP cells transfected with sh-NC or sh-NNT-AS1 or pcDNA or NNT-AS1. *P < 0.05