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Fig. 7 | Cancer Cell International

Fig. 7

From: LncRNA NNT-AS1 contributes to the cisplatin resistance of cervical cancer through NNT-AS1/miR-186/HMGB1 axis

Fig. 7

Knockdown of NNT-AS1 antagonizes DDP resistance in DDP-resistant CC cells via regulating HMGB1. a, b The mRNA and protein expression of HMGB1 in HeLa/DDP and SiHa/DDP cells transfected with sh-NC, sh- NNT-AS1, sh-NNT-AS1 + HMGB1 or sh-NNT-AS1 + pcDNA was measured using qRT-PCR or western blot, respectively. ce Cell viability and proliferation of HeLa/DDP and SiHa/DDP cells were detected by MTT assay. f The apoptosis rate was examined by Flow Cytometry in HeLa/DDP and SiHa/DDP cells. g, h The protein expression of cleaved caspase-3, Bcl-2 and Bax in HeLa/DDP and SiHa/DDP cells was detected by western blot. i, j Transwell assay was used to analyze migration and invasion abilities of HeLa/DDP and SiHa/DDP cells. k, l The levels of E-cadherin N-cadherin and Vimentin were detected by western blot. *P < 0.05

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