Fig. 6From: Nicotinamide N-methyltransferase inhibits autophagy induced by oxidative stress through suppressing the AMPK pathway in breast cancer cells1MNA inhibited autophagy induced by H2O2 by reducing the inhibition of cell viability and ATP production and the induction of ROS production. a The cell viability in SK-BR-3-Vector and MDA-MB-231-NNMTKD cells pretreated with and without 1MNA was assessed by MTS after H2O2 treatment. b The intracellular ROS level in SK-BR-3-Vector and MDA-MB-231-NNMTKD cells pretreated with and without 1MNA was assessed by flow cytometry after H2O2 treatment. c The intracellular ATP level in SK-BR-3-Vector and MDA-MB-231-NNMTKD cells pretreated with and without 1MNA was assessed by an ATP kit after H2O2 treatment. The SK-BR-3-Vector and MDA-MB-231-NNMTKD groups without 1MNA treatment were considered as the control groups, and their values were normalized to 100%. (*p < 0.05, and **p < 0.01) d The p-AMPK, p-ULK1, AMPK, ULK1 and LC3B protein levels in SK-BR-3-Vector and MDA-MB-231-NNMTKD cells pretreated with and without 1MNA were determined by Western blotting after H2O2 treatment. e Schematic model of this studyBack to article page