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Fig. 6 | Cancer Cell International

Fig. 6

From: Nicotinamide N-methyltransferase inhibits autophagy induced by oxidative stress through suppressing the AMPK pathway in breast cancer cells

Fig. 6

1MNA inhibited autophagy induced by H2O2 by reducing the inhibition of cell viability and ATP production and the induction of ROS production. a The cell viability in SK-BR-3-Vector and MDA-MB-231-NNMTKD cells pretreated with and without 1MNA was assessed by MTS after H2O2 treatment. b The intracellular ROS level in SK-BR-3-Vector and MDA-MB-231-NNMTKD cells pretreated with and without 1MNA was assessed by flow cytometry after H2O2 treatment. c The intracellular ATP level in SK-BR-3-Vector and MDA-MB-231-NNMTKD cells pretreated with and without 1MNA was assessed by an ATP kit after H2O2 treatment. The SK-BR-3-Vector and MDA-MB-231-NNMTKD groups without 1MNA treatment were considered as the control groups, and their values were normalized to 100%. (*p < 0.05, and **p < 0.01) d The p-AMPK, p-ULK1, AMPK, ULK1 and LC3B protein levels in SK-BR-3-Vector and MDA-MB-231-NNMTKD cells pretreated with and without 1MNA were determined by Western blotting after H2O2 treatment. e Schematic model of this study

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