Skip to main content
Fig. 5 | Cancer Cell International

Fig. 5

From: LINC00689 promotes prostate cancer progression via regulating miR-496/CTNNB1 to activate Wnt pathway

Fig. 5

LINC00689 activates Wnt pathway via upregulating CTNNB1. a The overexpression efficiency of CTNNB1 was measured by RT-qPCR and western blotting in pcDNA3.1/CTNNB1 transfected DU145 cells (48 h for plasmid transfection). b TOP/FOP flash assay detected the luciferase activity of β-catenin in DU145 cells under differently transfected conditions (sh-NC, sh-LINC00689#1 and sh-LINC00689#1 + pcDNA3.1/CTNNB1 and sh-LINC00689#1 + LiCL) (24 h post LiCL treatment). c, d RT-qPCR and western blot assays detected the mRNA and protein levels of Wnt-related proteins (CTNNB1/β-catenin, CCND1, CDK2 and c-MYC) in DU145 cells under differently transfected conditions (sh-NC, sh-LINC00689#1 and sh-LINC00689#1 + pcDNA3.1/CTNNB1 and sh-LINC00689#1 + LiCL) (24 h post LiCL treatment). GAPGH was an internal control. Error bars represent the mean ± SD of at least three independent experiments. **P < 0.01

Back to article page