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Fig. 2 | Cancer Cell International

Fig. 2

From: Oncogenic role of HMGA2 in fusion-negative rhabdomyosarcoma cells

Fig. 2

Effect of HMGA2 siRNA knockdown on FN-RMS cell growth. a RD, RMS-YM, and Rh18 cells were transfected with HMGA2 siRNA or negative control siRNA. Expression of HMGA2 was measured by quantitative RT-PCR and immunoblotting. Columns, mean of three independent experiments; bars, SD. * indicates statistical significance (P < 0.01; Student’s t-test). b Cell growth assay. RD, RMS-YM, and Rh18 cells were seeded in 24-well plates, cultured for 24 h, and then incubated with HMGA2 siRNA or negative control siRNA (day 0). Cells were harvested every 48 h, and nuclei were counted. Points, mean of three independent experiments; bars, SD. * indicates statistical significance (P < 0.01; Student’s t-test). c Cell cycle analysis was carried out by using siRNA-treated FN-RMS cells. RD, RMS-YM, and Rh18 cells were harvested and stained with propidium iodide and then analyzed for DNA content with FACSCalibur after siRNA transfection for 48 h, 24 h, and 24 h, respectively. Results represent the mean ± SD of three independent experiments (P < 0.01; Student’s t-test)

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