Fig. 4

miR-223 directly targets FBXW7 in NSCLC cells. a The predicted binding sequences of miR-223 in the 3′UTR of FBXW7 by TargetScan. b miR-223-3p mimics suppressed the luciferase activity of the wild-type but not mutant of FBXW7 reporter in 293T cells. **P < 0.01 compared with NC mimic + WT 3′UTR group. c Quantitative RT-PCR analyses of the effect of transient transfection with either the miR-223 mimic, miR-223 inhibitor, or negative control on the level of FBXW7 mRNA expression in NSCLC cells. *P < 0.05; **P < 0.01; ***P < 0.001 compared with control. d Quantification of FBXW7 protein expression in NSCLC cells transfected with either the miR-223 mimic, miR-223 inhibitor, or negative control by Western blot