Fig. 5

FBXW7 mediates miR-223 induced cisplatin-resistance and high levels of autophagy. a Quantitative RT-PCR analyses of the level of FBXW7 mRNA in NSCLC cells. b, c NSCLC cells were transfected with FBXW7 siRNA or the scrambled control. At 48 h post-transfection, different concentrations of cisplatin were added and cell viability was determined with a CCK-8 assay 24 h later and IC50 value was calculated. *P < 0.05; **P < 0.01 compared with control. d Immunoblot analyses of the effect of FBXW7 siRNA on NSCLC cells. e, f The cellular proliferation of NSCLC cells cultured in the IC50 of cisplatin following transfection with FBXW7 siRNA or the scrambled control detected via an Edu assay. Photomicrographs and bar charts depicted EdU staining and the relative EdU-positive ratio, respectively. *P < 0.05 compared with the NC + cisplatin group