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Fig. 5 | Cancer Cell International

Fig. 5

From: The novel long non-coding RNA LATS2-AS1-001 inhibits gastric cancer progression by regulating the LATS2/YAP1 signaling pathway via binding to EZH2

Fig. 5

LAST2-AS1-001 recruits EZH2 to upregulate LATS2, and promotes YAP1 phosphorylation. a RIP was performed to assess the interaction of LATS2-AS1-001 with EZH2. The enrichment of LATS2-AS1-001 with EZH2 was normalized to IgG. *P < 0.05. b The efficiency of EZH2 knockdown in BGC823 cells was assessed by qRT-PCR. c LATS2 mRNA levels were detected by qRT-PCR after EZH2 knockdown. LATS2 and YAP1 expression levels were measured by qRT-PCR and Western blot in BGC823 and SGC7901 cells overexpressing LAST2-AS1-001. The results showed that LATS-AS1-001 overexpression upregulated LATS2 (d, e, h), downregulated YAP1 (f–h) both at the mRNA and protein levels, and promoted YAP1 phosphorylation. In addition, the cell cycle related protein cyclin D1 was also downregulated after LATS-AS1-001 overexpression in GC cells (h)

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