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Fig. 1 | Cancer Cell International

Fig. 1

From: Knockdown of LINC00662 represses AK4 and attenuates radioresistance of oral squamous cell carcinoma

Fig. 1

LINC00662 attenuated radioresistance of OSCC cells. a qRT-PCR analysis of LINC00662 expression in OSCC (CAL27, SCC-4, SCC-1, TU183 and SCC-9) and normal human oral keratinocytes (NHOK) cell lines. b LINC00662 expression in CAL27 and SCC-4 cells under 4Gy irradiation was tested by qRT-PCR. c The expression of LINC00662 by transfection of sh-LINC00662#1 or sh-LINC00662#2 in CAL27 and SCC-4 cells was also measured by qRT-PCR. d CCK-8 assay was performed to examine cell viability of sh-LINC00662#2 transfected CAL27 and SCC-4 cells under 0 or 4Gy radiation, compared with relative control groups. e Survival fractions of sh-LINC00662#2 treated CAL27 and SCC-4 cells at the indicated doses of 0, 2, 4 and 8Gy radiation were respectively determined by colony formation assay. f Flow cytometry analysis of cell apoptosis in CAL27 and SCC-4 cells with LINC00662 down-regulation after 0 or 4Gy irradiation treatment. g Under 0 or 4Gy irradiation, cleaved PARP, cleaved caspase-3, total PARP and caspase-3 levels in CAL27 and SCC-4 cells with LINC00662 silencing were detected through western blot. *P < 0.05, **P < 0.01, and ***P < 0.001

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