Fig. 4

LINC00662 promoted AK4 mRNA stability through binding to hnRNPC protein. a AK4 expression levels in OSCC cell lines and NHOK were examined separately by qRT-PCR and western blot. b, c qRT-PCR and western blot results of the mRNA and protein levels of AK4 in sh-LINC00662#2 transfected CAL27 and SCC-4 cells. d qRT-PCR and western blot revealed the mRNA and protein levels of AK4 in LINC00662-KO transfected CAL27 and SCC-4 cells. e qRT-PCR revealed expression of LINC00662 and AK4 in pcDNA3.1-LINC00662 transfected CAL27 and SCC-4 cells. f Western blot assay revealed expression of AK4 by LINC00662 overexpression. g hnRNPC expression in CAL27 and SCC-4 cells transfected with pcDNA3.1-hnRNPC was measured through qRT-PCR and western blot assay. h With the addition of actinomycin D, qRT-PCR assessed the mRNA stability of AK4 in CAL27 and SCC-4 cells under the co-transfection of sh-LINC00662#2 and pcDNA3.1, or co-transfection of sh-LINC00662#2 and pcDNA3.1-hnRNPC. i RIP assays were carried out to test the impacts of LINC00662 and hnRNPC on the combination between hnRNPC protein and AK4. **P < 0.01 and ***P < 0.001