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Fig. 5 | Cancer Cell International

Fig. 5

From: Knockdown of LINC00662 represses AK4 and attenuates radioresistance of oral squamous cell carcinoma

Fig. 5

AK4 overexpression enhanced CAL27 and SCC-4 cell resistance to radiation. a The overexpression efficacy of AK4 in CAL27 and SCC-4 cells was detected by qRT-PCR and western blot assay. b CCK-8 assay was performed to examine cell viability of pcDNA3.1-AK4 transfected CAL27 and SCC-4 cells under 4Gy radiation. c Survival fractions of pcDNA3.1-AK4 treated CAL27 and SCC-4 cells at 4Gy radiation were determined by colony formation assay. dh Cell cycle, apoptosis, migration and invasion abilities were tested through flow cytometry, western blot and transwell assays in CAL27 and SCC-4 cells transfected with pcDNA3.1-AK4. **P < 0.01, and ***P < 0.001

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