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Fig. 3 | Cancer Cell International

Fig. 3

From: Circular RNA circ-CSPP1 regulates CCNE2 to facilitate hepatocellular carcinoma cell growth via sponging miR-577

Fig. 3

Circ-CSPP1 negatively regulated miR-577 expression. a The venn analysis on results from bioinformatics prediction software Starbase, Circular RNA Interactome and circbank. b Effects of circ-CSPP1 knockdown on the expression levels of predictive miRNAs were confirmed by qRT-PCR. c The binding sequences between miR-577 and circ-CSPP1. d The luciferase activity of cells. e RIP assay was performed to verify the interation between circ-CSPP1 and miR-577. f, g The subcellular distribution of circ-CSPP1 and miR-577 was examined by FISH. h Effects of circ-CSPP1 knockdown on the miR-577 expression level were confirmed by qRT-PCR. i MiR-577 expression in HCC tissues (HCC) and adjacent non-tumor tissues (Normal). j The correlation assay showed that miR-577 level was negatively correlated with circ-CSPP1 expression. *p < 0.05; **p < 0.01 and ***p < 0.001

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