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Fig. 4 | Cancer Cell International

Fig. 4

From: RETRACTED ARTICLE: Circular RNA circ-CSPP1 regulates CCNE2 to facilitate hepatocellular carcinoma cell growth via sponging miR-577

Fig. 4

Circ-CSPP1 positively regulated CCNE2 expression via miR-577. a Bioinformatics prediction software revealed that CCNE2 and TWF1 were targets of miR-577. b The mRNA expression levels of CCNE2 and TWF1 analyzed by qRT-PCR. c Western blotting showed the protein expression levels of CCNE2 and TWF1. d The binding sequences between miR-577 and CCNE2. e The luciferase activity of cells. f Effects of miR-577 mimics on the mRNA expression of CCNE2 was analyzed by qRT-PCR. g Western blotting showed the protein expression levels of CCNE2. (H) CCNE2 expression in HCC tissues (HCC) and adjacent non-tumor tissues (Normal). i The correlation assay showed that CCNE2 level was negatively correlated with circ-CSPP1 expression. j The correlation assay showed that CCNE2 level was positively correlated with circ-CSPP1 expression. *p < 0.05; **p < 0.01 and ***p < 0.001

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