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Fig. 2 | Cancer Cell International

Fig. 2

From: HMGB1 mediates lipopolysaccharide-induced inflammation via interacting with GPX4 in colon cancer cells

Fig. 2

HMGB1 regulates inflammation via ROS-mediated. a To investigate whether ROS was involved in inflammation, we detected the ROS levels in SW480 and HCT116 cells and the results demonstrated that LPS could promote ROS accumulation. b The cells treated with ROS scavenger NAC and harvested for western blot, the results showed that NAC inhibited the protein levels of p-IKBα and p-p65 stimulated by LPS. c In cells treated with siHMGB1 to investigated ROS levels, siHMGB1 significantly suppressed the ROS accumulation. d The levels of GSH were detected and the results showed that LPS effectively repressed the GSH levels. In contrast, siHMGB1 increased the GSH levels. ROS, reactive oxygen species; NAC, N–acetyl–cysteine; GSH, Glutathione

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