Fig. 4

Effects of LPS on HMGB1 translocation. a Western blot were performed to observe HMGB1 translocation and the results showed that LPS increased HMGB1 expression in cytoplasm but decreased the nuclear HMGB1 protein levels in SW480 and HCT116 cells. b When inhibitor Gly of HMGB1 was introduced, western blot results demonstrated that HMGB1 translocation was blocked. c IP results revealed that Gly attenuated the interaction between HMGB1 and GPX4. We investigated whether Gly have an impact on ROS levelsand NF-kB and the results showed that Gly significantly suppressed the ROS levels (d) and p-IKBα and p-p65 protein levels (e) increased by LPS. Gly, Glycyrrhizic acid