Fig. 4

SNHG16 deletion impairs cell cisplatin resistance and malignant phenotypes by sponging miR-338-3p in neuroblastoma. SK-N-AS-R and SK-N-SH-R cells were transfected with si-NC, si-SNHG16, si-SNHG16 + in-miR-NC, si-SNHG16 + in-miR-338-3p. a The relative expression of miR-338-3p was measured using qRT-PCR. b The IC50 value for cisplatin was assessed by CCK-8 assay. c Western blot assay was used to detect the expression of drug-resistance associated proteins MRP1 and P-gp. d, e Cell proliferation was examined using CCK-8 assay. f, g Transwell assay was used for the detection of cell migration and invasion ability. h Cell apoptosis was analyzed using Flow cytometric analysis. Each experiments were repeated three times independently, and the average was taken. *P < 0.05