Fig. 6

PLK4 overexpression reverses miR-338-3p re-expression-mediated inhibition on cisplatin resistance and tumorigenesis in neuroblastoma cells. SK-N-AS-R and SK-N-SH-R cells were transfected with miR-NC, miR-338-3p, miR-338-3p + pcDNA, or miR-338-3p + PLK4. a Western blot was used to detect the expression of PLK4. b The IC50 value for cisplatin was calculated by CCK-8 assay. c The expressions of drug-resistance associated proteins MRP1 and P-gp were examined by western blot assay. d, e The CCK-8 assay was performed to detect cell proliferation. f, g Transwell assay was used to determine cell migration and invasion ability. h The apoptosis rate was analyzed using Flow Cytometry. Each experiment was performed for three times, and results represented as the average of three independent replicates. *P < 0.05