Fig. 6

HOXD3 knockdown reversed the promoting effects of anti-miR-345-5p on cell progression in matrine-induced HCC cells. After Huh-7 and HCCLM3 cells were exposed to different treatments (control, Mat, Mat + anti-miR-NC, Mat + anti-miR-345-5p, Mat + anti-miR-345-5p + si-NC and Mat + anti-miR-345-5p + si-HOXD3). a, b The mRNA and protein levels of HOXD3 were measured by qRT-PCR and western blot, respectively. c, d Cell viability and apoptosis were examined through CCK-8 assay and Flow cytometry, respectively. e, f Western blot assay was used to measure the levels of Bcl-2, Bax and cleaved-caspase 9. g–j Cell cycle, migration and invasion were assessed by Flow cytometry and Transwell assay, respectively. k–l Western blot assay was performed to evaluate the levels of LC3-II, LC3-I and Beclin 1. *P < 0.05