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Fig. 6 | Cancer Cell International

Fig. 6

From: Matrine inhibits cell growth, migration, invasion and promotes autophagy in hepatocellular carcinoma by regulation of circ_0027345/miR-345-5p/HOXD3 axis

Fig. 6

HOXD3 knockdown reversed the promoting effects of anti-miR-345-5p on cell progression in matrine-induced HCC cells. After Huh-7 and HCCLM3 cells were exposed to different treatments (control, Mat, Mat + anti-miR-NC, Mat + anti-miR-345-5p, Mat + anti-miR-345-5p + si-NC and Mat + anti-miR-345-5p + si-HOXD3). a, b The mRNA and protein levels of HOXD3 were measured by qRT-PCR and western blot, respectively. c, d Cell viability and apoptosis were examined through CCK-8 assay and Flow cytometry, respectively. e, f Western blot assay was used to measure the levels of Bcl-2, Bax and cleaved-caspase 9. gj Cell cycle, migration and invasion were assessed by Flow cytometry and Transwell assay, respectively. kl Western blot assay was performed to evaluate the levels of LC3-II, LC3-I and Beclin 1. *P < 0.05

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