Fig. 4

GSK-J4 induces cell cycle arrest via ER stress. a Pre-treatment with 4-PBA (3 mM) can alleviate the accumulation of cells in the S phase. b The quantitative cell cycle distribution data. Values represent the mean ± SD of three independent experiments. **p < 0.01. c. After pre-treatment with 4-PBA (3 mM), the expression levels of P21, CyclinD1 and CyclinA2 in KG-1a cells were detected by Western blotting. d–f Statistical analysis of the expression levels of P21, CyclinD1 and CyclinA2 in KG-1a cells exposed to 4-PBA, GSK-J4 and their combination. β-Actin was used as an internal control. Values represent the mean ± SD of three independent experiments.*p < 0.05, **p < 0.01 compared to the control group