Fig. 1

miR-454 overexpression inhibits the proliferation, migration and invasion of ovarian cancer cells. a Expression of miR-454 was examined in OVCAR3 and SKOV3 cells under hypoxic culture conditions (1% O₂, 5% CO₂, 94% N₂) for 6 or 24 h. b Expression of miR-454 in the serum of ovarian cancer patients (n = 13) and normal samples (n = 6) was measured by RT-PCR. c Expression of miR-454 in OVCAR3 and SKOV3 cells transfected with either pCMV-MIR-miR-454 or pCMV-MIR plasmid was detected byRT-PCR. d, e CCK8 assay was used to examine the proliferation of OVCAR3 (d) and SKOV3 (e) cells after transfected with either pCMV-MIR-miR-454 or pCMV-MIR plasmid. f Colony formation assay of OVCAR3 and SKOV3 cells with stable miR-454 overexpression. g, h Transwell assay was performed to assess the migration (g) and invasion (h) abilities of OVCAR3 and SKOV3 cells with stable miR-454 overexpression. NC, cells transfected with pCMV-MIR plasmid; miR-454, cells transfected with pCMV-MIR-miR-454 plasmid. *P < 0.05, **P < 0.01 vs. NC group