Fig. 3From: miR-454 suppresses the proliferation and invasion of ovarian cancer by targeting E2F6E2F6 is a direct target of miR-454 in ovarian cancer cells. a The predicted binding site for miR-454 in the 3′-UTR of E2F6 mRNA was revealed by TargetScan. b Relative luciferase activity in cells co-transfected with pCMV-MIR-miR-454 or pCMV-MIR and pmir-GLO-E2F6 wild type (wt) or pmir-GLO-E2F6 mutant (mut) was measured by dual-luciferase reporter assay. c The protein expression of E2F6 was checked in OVCAR3 and SKOV3 cells transfected with either pCMV-MIR-miR-454 or pCMV-MIR plasmid by western blot. **P < 0.01 vs. NC groupBack to article page