Fig. 4

LINC01094 enhanced FOXM1 protein expression in ccRCC via miR-577/CHEK2 signaling. a qRT-PCR analysis of CHEK2 level in LINC01094-inhibited ccRCC cells. b Luciferase reporter assay confirmed the competitive binding of LINC01094 and CHEK2 mRNA with miR-577. c Co-existence of LINC01094, miR-577 and CHEK2 mRNA in RISC was validated through RIP assay. d The expression level of CHEK2 in indicated ccRCC cells was examined via qRT-PCR. e, f The expression of FOXM1 at mRNA and protein levels in Caski and 786-O cells with or without LINC01094 suppression was determined by qRT-PCR and western blot analyses. g Transfection efficiency of pcDNA3.1/CHEK2 and pcDNA3.1 was tested by qRT-PCR. h, i The mRNA and protein expression of FOXM1 in indicated ccRCC cells was detected via qRT-PCR and western blot. *P < 0.05, **P < 0.01, ***P < 0.001