Fig. 1

ZKSCAN3 is frequently overexpressed in HCC tissues and cell lines. a Relative ZKSCAN3 mRNA expression levels in HCC tissues and matched adjacent nontumor tissues were determined by qRT-PCR. b Representative Western blot analysis of ZKSCAN3 expression in the HCC (T) and nontumor tissues (NT) was shown. c Representative images of IHC staining of ZKSCAN3 in HCC and adjacent non-tumor tissues. Comparing differences in the expression level of ZKSCAN3 protein d between HCC cell lines with different metastatic potentials and the immortalized normal hepatic cell LO2. e HCC patients with higher expression of ZKSCAN3 had worse overall survival and disease-free survival. f 182 cases with high-/low-expression) were analyzed. n = three repeats with similar results; *P < 0.05