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Fig. 4 | Cancer Cell International

Fig. 4

From: ZKSCAN3 drives tumor metastasis via integrin β4/FAK/AKT mediated epithelial–mesenchymal transition in hepatocellular carcinoma

Fig. 4

ZKSCAN3 induces integrin β4 expression via directly binding to its promoter. a qRT-PCR and b Western blot showed that ZKSCAN3 overexpression promoted ITGB4 mRNA (a) and protein (b) expression, while ZKSCAN3 knockdown inhibited ITGB4 mRNA (a) and protein (b) expression. c Positive association between ZKSCAN3 and ITGB4 expression in HCC tissues. d Data from GEPIA database showed that ZKSCAN3 expression has a positive correlation with the expression of ITGB4. e ChIP assay. Anti-IgG and anti- ZKSCAN3 antibodies were used in the ChIP assay. f Luciferase analysis in Hep3B cells. Hep3B cells overexpressing ZKSCAN3 expression plasmid or control vector were transfected with the pGL3-ITGB4 promoter and pGL3-ITGB4-promoter mutation. 48 h after transfection, cells were subjected to dual luciferase analysis. Results were expressed as a fold induction relative to the cells transfected with the control vector after normalization to Renilla activity. g, h ITGB4 shRNA was transfected into Hep3B cells to knockdown ITGB4 in ZKSCAN3-overexpressing cells. Transwell assay for migration and invasion (g) and western blot (h) for EMT markers were performed. *P < 0.05

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