Fig. 4

Knockdown of circ_0000284 impeded tumorigenesis of NSCLC in vitro and in vivo. A549 and H82 cells were severally transfected with sh-NC or sh-circ_0000284. a–d The measurement of cell proliferation (a), colony formation (b) and migration/invasion (c, d) was implemented with respective CCK-8, colony formation assay and transwell assay. e The proliferation and metastasis-related proteins were determined using western blot. f, g The volume (f) and weight (g) of tumors in sh-NC and sh-circ_0000284 groups were measured. h Circ_0000284 and HIPK3 mRNA expression levels in tumors of mice were analyzed by qRT-PCR. All experiments were independently performed three times with N value = 3. *P < 0.05