Fig. 3

circUBAP2 interacted with and negatively regulated miR-641 in OS cells. a The putative binding sites between circUBAP2 and miR-641 were predicted by starBase. b The luciferase activity in OS cells cotransfected with miR-641 and circUBAP2 WT or circUBAP2 MUT was checked. c, d The level of miR-641 in OS tissues and cells was evaluated by qRT-PCR. e The correlation between circUBAP2 and miR-641 in OS tissues was determined using Pearson’s correlation coefficient. f The expression of circUBAP2 in OS cells transfected with OE-circUBAP2, as well as matched controls, was checked by qRT-PCR. g The level of miR-641 in Control and OS cells transfected with si-NC, si-circUBAP2, Vector or OE- circUBAP2 was measured by qRT-PCR. *P < 0.05