Fig. 6

MiR-641 bound to the 3′UTR of YAP1 and its impacts on proliferation and invasion were transposed by YAP1 overexpression. a The putative target sites between miR-641 and YAP1 were predicted by starBase. b The dual-luciferase reporter assay was performed to corroborate the interaction between miR-641 and YAP1. c The protein level of YAP1 in Control and OS cells infected with Anti-miR, miR-641 or matched controls was detected by western blot. d The protein level of YAP1 in Control and OS cells infected with Vector or OE-YAP1 was measured by western blot. e Cell proliferation at different time points was checked by CCK-8 assay. f Transwell assay was hired to check cell invasion and corresponding invaded cells were counted. g, h The protein levels of E-cadherin and Vimentin in Control and infected OS cells were determined by western blot. *P < 0.05