Fig. 4

MiR-324-3p targeted SUDS3 in TNBC. a The possible mRNAs bound to miR-324-3p were predicted by PITA, microT, PicTar and miRmap databases. b The expression levels of SELENOW, ZBTB34, SUDS3 and STAG2 were tested through qRT-PCR in TNBC cells and MCF-10A cells. c RIP experiment was utilized to test the co-harvest of SNHG22, miR-324-3p and SUDS3 in RISCs. d The binding sites between miR-324-3p and SUDS3 provided by ENCORI. e, f Luciferase reporter and RNA pull down experiments were conducted to verify the correlation between miR-324-3p and SUDS3. g, h The expression of SUDS3 was tested by qRT-PCR and western blot when miR-324-3p was overexpressed or SNHG22 was inhibited in MDA-MB-231 and MDA-MB-468 cells. i The interference efficiency of miR-324-3p was detected through qRT-PCR in MDA-MB-231 and MDA-MB-468 cells. j The expression of SUDS3 was tested through qRT-PCR and western blot in MDA-MB-231 and MDA-MB-468 cells with inhibited SNHG22 or together with suppressed miR-324-3p. **P < 0.01