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Fig. 4 | Cancer Cell International

Fig. 4

From: LncRNA ZFAS1 promotes pancreatic adenocarcinoma metastasis via the RHOA/ROCK2 pathway by sponging miR-3924

Fig. 4Fig. 4

ZFAS1 functions as a sponge of miR-3924. a The flowchart of all bioinformatic analyses. b The binding site of ZFAS1/miR-3924 predicted by DIANA-LncBase 2.0. c Venn diagram of the target miRNAs predicted with starBase 3.0 and DIANA-LncBase 2.0. d HEK-293T cells transfected with ZFAS1 3′-UTR Wt, Mut, NC or PC reporter plasmids along with miR-3924 mimics. Relative luciferase activity was measured (n = 3), *p < 0.05. eg Wound healing assays showing that miR-3924 inhibition reversed the effects of ZFAS1 silencing (n = 3), *p < 0.05; scale bars, 50 μm. h, i Transwell migration assays showing that miR-3924 inhibition reversed the effects caused by ZFAS1 silencing (n = 3), *p < 0.05; scale bars, 50 μm. j Relative ZFAS1 expression in cells transfected with sh-NC or sh-ZFAS1 was measured by qRT-PCR (n = 3), *p < 0.05. k, l Wound healing assays showing that miR-3924 overexpression inhibited the metastasis of BXPC3 and SW1990 cells (n = 3), *p < 0.05; scale bars, 50 μm. m Transwell migration assays showing that miR-3924 overexpression inhibited the migration of BXPC3 and SW1990 cells (n = 3), *p < 0.05; scale bars, 50 μm

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