Fig. 4

Knockdown of KIF21B promotes apoptosis in NSCLC cells. a After being infected with sh-KIF21B lentivirus, Hoechst 33342/PI assay was used to assess cell apoptosis in H1299 and A549 cells. The scale bar was 100 μm. b The expression of apoptosis-related proteins (Bcl-2, Bax and active Caspase 3) was detected by western blot in H1299 and A549 cells after indicated transfection. c The expression of apoptosis-related proteins was detected by western blot in BEAS-2B cells. d The expression of key components of the Akt signaling pathway (Akt, p-Akt and Cyclin D1) was examined using western blot in H1299 and A549 cells. e The expression of key components of the Akt signaling pathway was examined using western blot in BEAS-2B cells. Data are presented as the mean ± standard deviation. Results were obtained in three replicates. *P < 0.05