Fig. 2

Silencing of circ_LDLR inhibited PTC cell colony formation, proliferation, migration and invasion and induced cell apoptosis. TPC-1 and SW579 cells were transfected with si-NC or si-circ_LDLR. a The expression of circ_LDLR in TPC-1 and SW579 cells was examined using qRT-PCR. b The colony formation of TPC-1 and SW579 cells was assessed by colony formation assay. c, d The proliferation of TPC-1 and SW579 cells was evaluated using MTT assay. e The protein level of Ki67 in TPC-1 and SW579 cells was measured by western blot assay. f The apoptosis of TPC-1 and SW579 cells was analyzed by flow cytometry analysis. g, h The migration and invasion of TPC-1 and SW579 cells were evaluated by transwell assay. i, j The protein levels of Twist1 and E-cadherin in TPC-1 and SW579 cells were measured through western blot assay. *P < 0.05. Each bar represents mean ± SD