Fig. 4

MiR-195-5p inhibition reversed the inhibitory effect of circ_LDLR knockdown on PTC cell progression. TPC-1 and SW579 cells were treated with si-NC, si-circ_LDLR, si-circ_LDLR + anti-miR-NC or si-circ_LDLR + anti-miR-195-5p. a MiR-195-5p expression level in TPC-1 and SW579 cells was measured by qRT-PCR. b Cell colony formation ability in TPC-1 and SW579 cells was assessed by colony formation assay. c, d Cell proliferation in TPC-1 and SW579 cells was evaluated by MTT assay. e The protein level of Ki67 in TPC-1 and SW579 cells was examined using western blot assay. f Cell apoptosis in TPC-1 and SW579 cells was analyzed using flow cytometry analysis. g, h Cell migration and invasion in TPC-1 and SW579 cells were determined by transwell assay. i, j The protein levels of Twist1 and E-cadherin in TPC-1 and SW579 cells were detected through western blot assay. *P < 0.05. Each bar represents mean ± SD