Fig. 5

LIPH was identified as a target gene of miR-195-5p in PTC cells. a The targeted genes of miR-195-5p analyzed by StarBase 3.0 and GEO dataset GSE54958. b, c The mRNA and protein levels of LIPH, GALNT7, PSD3 and ITGA2 in Nthy-ori3-1, TPC-1 and SW579 cells were measured by qRT-PCR assay and western blot assay. d The potential biding sites between the 3′UTR of LIPH and miR-195-5p were presented. e, f The targeting relationship between miR-195-5p and LIPH was investigated by dual-luciferase reporter assay. g–i MiR-NC, miR-195-5p, anti-miR-NC or anti-miR-195-5p was transfected into TPC-1 and SW579 cells and then the levels of miR-195-5p, LIPH mRNA and LIPH protein were detected using qRT-PCR assay or western blot assay. j The mRNA expression of LIPH in PTC and normal tissues (N = 60) was determined using qRT-PCR (Student’s t-test).. k, l The correlations between miR-195-5p and LIPH mRNA, as well as LIPH mRNA and circ_LDLR in PTC tissues were analyzed by Spearman’s correlation coefficient analysis. m The protein level of LIPH in PTC tissues and normal tissues was measured using western blot assay. n, o TPC-1 and SW579 cells were transfected with si-NC, si-circ_LDLR, si-circ_LDLR + anti-miR-NC or si-circ_LDLR + anti-miR-195-5p and then the mRNA and protein levels of LIPH were detected through qRT-PCR assay and western blot assay, respectively. *P < 0.05. Each bar represents mean ± SD