Fig. 10

MiR-1269a inhibited the metastasis of LC cells through targeting FOXO1. a–c The migration of A549 and SPC-A1 cells was determined by wound-healing assay (magnification: 100x). The migration of A549 and SPC-A1 cells was calculated (b and c). (d–f) The invasion of A549 and SPC-A1 cells was calculated (d and e). The invaded cells (f) were subjected to transwell assay and shown under a microscope (magnification: 200x). ^*P < 0.05, ^**P < 0.001, vs Vector. ^#P < 0.05, ^##P < 0.05 vs. FOXO1. ^{^}P < 0.05, ^{^^}P < 0.05 vs. mimic. Vector: pCDH-CMV empty plasmid. FOXO1: pCDH-CMV-FOXO1 overexpression plasmid. Mimic: miR-1269a mimic. FOXO1 + mimic: pCDH-CMV-FOXO1 and miR-1269a mimic. All experiments repeated independently in triplicate