Fig. 4

LINC00261 overexpression inhibited the migration, invasion and related protein expressions in LC cells. a–c The migration of A549 and SPC-A1 cells was determined by wound-healing assay a. Magnification: 100×. The migration of A549 and SPC-A1 cells was calculated (b, c). d–f The invasion of A549 and SPC-A1 cells was calculated (d, e). The invaded cells (f) were subjected to transwell assay and observed under a microscope (Magnification: 200x). g–j The expression levels of MMP-3 and MMP-9 were determined by Western blot. GAPDH served as an internal control. ^**P < 0.001 vs. blank, ^##P < 0.001 vs NC. Blank: cells treated with transfection regent only. NC: pCDH-CMV empty plasmid. LINC00261: pCDH-CMV-LINC00261 overexpression plasmid. All experiments repeated independently in triplicate