Fig. 6

MiR-1269a targeted FOXO1 mRNA and was sponged by LINC00261. a A potential binding site of miR-1269a at LINC00261 predicted by starBase. b, c The relative luciferase activities of A549 and SPC-A1 cells were determined by dual- luciferase reporter assays. d A potential binding site of miR-1269a at position 65–72 of FOXO1 3′UTR predicted by Targetscan7.2. e, f The relative luciferase activities of A549 and SPC-A1 cells were determined by dual-luciferase reporter assays. ^**P < 0.001 vs. blank. LINC00261-WT: LINC00261-WT luciferase reporter plasmid. LINC00261-MUT: LINC00261-MUT luciferase reporter plasmid. FOXO1-WT: FOXO1 3′UTR-WT luciferase reporter plasmid. FOXO1-MUT: FOXO1 3′UTR -MUT luciferase reporter plasmid. All experiments repeated independently in triplicate