Fig. 1
From: A novel chalcone derivative suppresses melanoma cell growth through targeting Fyn/Stat3 pathway
Fyn is overexpressed in melanoma cells and knockdown of Fyn induces apoptosis in melanoma cells. a Knocked down of Fyn with two independent shRNA in melanoma cells Sk-Mel-5 and Sk-Mel-28, the expression of Fyn was assessed by western blotting with anti-Fyn antibody. b Cell viability of knocked down of Fyn in melanoma cells Sk-Mel-5 and Sk-Mel-28 were tested by CCK-8 assay. All samples represented at least for four replicates with three independent experiments, ****P < 0.0001. c Apoptosis was determined by flow cytometry with Annexin V and PI double staining in Fyn knocked down melanoma cells Sk-Mel-5 and Sk-Mel-28, data was presented as mean (n = 3) ± SD, **P < 0.01, ***P < 0.001, ****P < 0.0001. d Knocked down of Fyn in melanoma cells Sk-Mel-5 and Sk-Mel-28, Cell lysates were quantified and separated by SDS-PAGE. Western blot of the indicated proteins was done as described in “Methods”. Specific antibodies were used for detection of indicated proteins and a-tubulin was used as an internal control